Laboratorio de Ecotoxicología Acuática, Junín de los Andes, Neuquén, Argentina . likes · 2 talking about this · 1 was here. Laboratorio de. Laboratorio de Ecotoxicología Acuática, Junín de los Andes, Neuquén, Argentina . likes · 1 was here. Laboratorio de Ecotoxicología Acuática (LEA). Laboratorio de Ecotoxicología Acuática in Junín De Los Andes – Contact details, Address Map, Photos, offers, Real time Reviews and Ratings.

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Effects on circulating hemocyte integrity and immune related functions were mostly transient or remained stable within 7 days; except for increased lysosomal labialization and decreased extracellular ROS production when mussels were exposed to the toxin combination.

Since oil exploration and extraction activities are expected to be enhanced in this region in the future, further studies and monitoring programs are needed acuwtica protect the quality of valuable water ecosystems, fish production in downstream reservoirs and human health. Since direct measurement of tissue hydrocarbon concentrations generally does not provide a useful indicator of petroleum exposure in fish and could be expensive Collier et al.

Biomarker induction in tropical fish species on the northwest shelf of Australia by produced formation water.

Nuclear receptor-mediated toxicity of xenobiotics. However, there are few studies on the nature of PXR ligands in fish Ekins et al. Lysosomal membrane destabilization were more closely, but not exclusively, matched with LC exposure.

A cycloheximide-sensitive factor regulates TCDD-induced degradation of the aryl hydrocarbon receptor. The only effect attributable exclusively to PST was increased phagocytic activity after 3 days and impaired feeding activity after 7 days, which curtailed toxin accumulation in digestive glands.

Bands were analyzed densitometrically using Image J software. The number of cycles of denaturation phase was selected to catch the exponential amplification, by previous calibration of each mRNA. The evolution of farnesoid X, vitamin D, and pregnane X receptors: One of these old wellbores is located near to Bariloche city, North West Patagonia, and was under production from up to the s, when it was dismantled leaving a permanent heavy oil spill Mancini and Serna Cytochrome P CYP gene expressions were studied by semi-quantitative polymerase chain reaction PCR to assess oil pollution using liver and gills of juvenile rainbow trout Oncorhynchus mykiss sampled from La Mina stream Bariloche, Argentinaup and downstream of a discharge of petroleum spill from an unproductive wellbore.


We performed post hoc comparisons between control and treatments, for both 24 and 96 h, of all pairs of groups using two-sided significance levels with a Bonferroni adjustment. A Oil remains afloat on the surface of stream water. CYP1A mRNA expression is induced by minuscule amounts of diverse aromatic compounds through the aryl hydrocarbon receptor AhR signaling pathway, being a sensitive biomarker of exposure to aromatic hydrocarbon contaminants Uno et al.

For the experiments, juvenile rainbow trout 3. Hence, CYP1A transcript expression serves as reference in biomarkers of crude oil pollution. We focused on gill and liver tissues because the gills are the primary route of hydrocarbon uptake from water, and the liver is the main organ for metabolism of toxic oil constituents Thomas and Rice Induction patterns of new CYP1 genes in environmentally exposed rainbow trout.

Mar del Plata, Argentina, Aprilpp. Comparison of rainbow trout and mammalian cytochrome P enzymes: In order to test if orally supplied Euglena sp. Data was complemented with laboratory exposure to water accommodated fraction WAF from the oil spill.

In vitro effects of endosulfan-based insecticides on mammalian sperm.

This explains the similar response in liver CYP1A transcript levels at 24 and 96 h during our experiments, without dose-dependent effects. Chemosphere 46 Wild, juvenile rainbow trout Gene transcript expression of several cytochrome P oxidases was studied by semi-quantitative PCR in both field and laboratory. The TPH was extracted from each with carbon tetrachloride and was estimated by the analysis of the triple peak method EPA at three spectral regions: CYP3A27 levels were only induced in liver of wild trout 1.

Although the results in the WAF experiment are not conclusive, differences among time response of CYP2 expression could be useful as complementary indicators of pollution by crude oil. An integrated biomarker-based strategy for ecotoxicological evaluation of risk in environmental management.

However, in the early 20th century, the black gold fever had already impacted some Patagonian environments in several ways, such as the footprint of old unproductive wellbores. Fish have proved to be useful models when it comes to evaluating the health of aquatic ecosystems.

In vitro effects of endosulfan-based insecticides on mammalian sperm.

Masaryk University, Czech Republic, 62 pp. Samples were diluted with ultra-pure water to adjust to a concentration of 2. CYP2K1 is the most abundant constitutively expressed hepatic cytochrome P found in sexually immature rainbow trout and it shows an unusually high sensitivity to carcinogens Bailey et al.


The phase I xenobiotic metabolizing enzymes are represented mainly by cytochrome P CYP multi-enzymatic complex, that catalyzes hydrolysis, oxidation and reduction reactions, by adding or exposing functional groups Danielson The upstream reach is up of a 1. Functional characterization of a full length pregnane X receptor, expression in vivo, and identification of PXR alleles, in Zebrafish Danio rerio. These clear and consistent changes reaffirm CYP1A transcript expression as a good semiquantitative biomarker for crude oil exposure.

Julieta Soledad De Anna. Hence, the response of the last ones to crude oil could be less specific but not less useful indicating some metabolic alterations.

The transcript levels of CYP2M1 declined 1. Standardization of the preparation and quantitation of water-accommodated fractions of petroleum for toxicity testing. Cloning, tissue distribution, and functional studies of a new cytochrome P 3A subfamily member, CYP3A45, from rainbow trout Oncorhynchus mykiss intestinal ecotoxicologla.

Tested parameters comprised signs of paralysis, feeding activity, and immune cell integrity hemocyte numbers and viability; lysosomal membrane destabilization and function ROS production. Triplicate samples were collected at the upstream reach —, — and — mat the oil spill zero metersat the downstream reach 50, and m and further downstreamand m.

The cytochrome P superfamily: Rainbow trout Oncorhynchus mykiss is a useful model for toxicology studies because of its sensitivity to chemicals Bailey et al.

As far as we know, this is the first ecotoxicological study on the oil spill in La Mina stream, and one of the few related studies in Patagonia. Hence, patterns of multiple CYP genes expression in rainbow trout gills and liver could be especially valuable for monitoring programs of crude oil pollution and remediation actions on Patagonian freshwater ecosystems.

Petroleum exposure and associated biochemical effects in subtidal fish after the Exxon Valdez oil spill. Fish are represented by a single species, the exotic rainbow trout O. Lysosomal membrane stability is higher in EG mussels than in SV ones for all conditions, although it is decreased at polluted sites compared with that at C.

Anchorage Alaska, Februarypp.